Tuesday, July 23, 2013

Introducing: ScriptSeq™ Complete Gold Kit (Yeast)


Introducing: ScriptSeq™ Complete Gold Kit (Yeast)
The ScriptSeq™ Complete Gold Kit (Yeast) combines Epicentre's powerful Ribo-Zero™ technology and rapid ScriptSeq v2 RNA-Seq library prep procedure for optimized comprehensive preparation of RNA-Seq libraries in 1 day (Fig. 1). The kit requires 1-5 µg of yeast total RNA and contains Ribo-Zero rRNA removal reagents for removal of 25/28S,18S, 5.8S, and 5S rRNA along with 21S-mt and 15S-mt rRNA from yeast total RNA, along with the ScriptSeq v2 RNA-Seq kit for directional RNA-Seq.


Figure 1. An overview of the ScriptSeq™ Complete Gold Kit (Yeast) process. Cluster-ready ScriptSeq RNA-Seq libraries, virtually rRNA-free, are made in 1 day from as little as 100 ng of intact or partially degraded total RNA.

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