Friday, February 22, 2013

MasterPure™ Yeast DNA used in detection of fungal meningitis

Fungal meningitis is a catastrophic infectious disease and was recently attributed to injections of contaminated methylprednisolone, which is ongoing in the U.S. The major cause of this meningitis is Exserohilum rostratum, a mold that is rarely recognized as a human pathogen. In the infectious process, the fungus has been identified from patient tissue and cerebrospinal fluid (CSF) samples, as well as unopened vials of the implicated lots of methylprednisolone, by culture or DNA sequencing, followed by a PCR assay.  Zhao et al. used the MasterPure™ Yeast DNA Purification Kit for isolation of gDNA for the development of a PCR assay to diagnose these fungal infections. The authors isolated the pathogen from patient tissue samples and CSF, cultured the fungus for 5 days on potato dextrose agar, and removed a small portion of the mycelial mat. Genomic DNA was isolated using the MasterPure Yeast DNA Purification Kit. The ability to quickly and easily isolate fungal DNA was instrumental  in developing a sensitive, rapid qPCR “Molecular beacon” assay using a specific E. rostratum probe. This assay is used for  diagnosing and tracing infected medications and can be used as a method for disease-monitoring and quality-checking the sterility of medications as well as monitoring the progress of treatment in infected patients.

ResearchBlogging.orgZhao Y, et al (2013). A Real-Time PCR Assay for Rapid Detection and Quantification of Exserohilum rostratum, a Causative Pathogen of Fungal Meningitis Associated with Injection of Contaminated Methylprednisolone. Journal of clinical microbiology, 51 (3), 1034-6 PMID: 23303500

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