Wednesday, December 12, 2012

New Product Announcement: ARTseq™ Ribosome Profiling Kits

ARTseq™ Ribosome Profiling Kits provide a ‘snapshot’ of the ribosomes active in a cell at a specific time point by sequencing only the ribosome-protected mRNA fragments. This powerful method can be used to investigate translational control, measure gene expression, and predict protein abundance. ARTseq is available for mammalian and yeast cell lines.

New Product Announcement: Ribo-Zero™ Magnetic Gold Kit (Epidemiology)

Remove rRNA from human or rodent samples mixed with bacteria in two hours with Epicentre’s Ribo-Zero™ Magnetic Gold Kit (Epidemiology). The kit has been designed to deplete a wide range of sample mixtures, so you do not need to know how much of your sample is bacterial or human (rodent).  Total RNA input of ≥ 100 ng is successfully depleted and converted into a sequencing library for the Illumina® platforms in less than a day using ScriptSeq™ Complete Gold Kit (Epidemiology) - Low Input. Higher total RNA amounts of ≥ 500 ng are perfect for ScriptSeq Complete Gold Kit (Epidemiology).

The simple protocol is perfect for human or animal disease studies. A paper recently published by San Diego State University highlights removal efficacy, and subsequent enrichment of coding and noncoding RNA, in sputum from patients living with cystic fibrosis.

ResearchBlogging.orgLim YW, et al. (2012). Metagenomics and metatranscriptomics: Windows on CF-associated viral and microbial communities. Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society PMID: 22951208