The results showed that massive genomic restructuring appears to be a catalyst for development of the DFTD syndrome, based on chromosome painting and fluorescence in situ hybridization (FISH) experiments conducted using the BAC library. The authors developed a detailed map of the global chromosome restructuring and intricate gene rearrangements that characterize DFTD. Only limited regions of the genome were found to be highly rearranged. After the rearrangements occur, the tumor karyotype is remarkably stable during its clonal transmission from animal to animal. By anchoring genes to a reference and tumor maps, the authors believe that they can predict the locations of common tumor suppressor genes and oncogenes. This study provides an important framework for future genomic studies into DFTD and enhances the value of the creation of large-insert genomic libraries.
Deakin, J. et al. (2012). Genomic Restructuring in the Tasmanian Devil Facial Tumour: Chromosome Painting and Gene Mapping Provide Clues to Evolution of a Transmissible Tumour PLoS Genetics, 8 (2) DOI: 10.1371/journal.pgen.1002483