The authors state that no chemically modified probes or primers are required for their method. The key elements of the miR-ID process (miRNA circularization, reverse transcription of circularized RNA, and qPCR using 5’-overlapping primers) are likely to be of general interest in other transcriptome discovery techniques.
Kumar, P. et al. (2010). miR-ID: A novel, circularization-based platform for detection of microRNAs RNA, 17 (2), 365-380 DOI: 10.1261/rna.2490111