The researchers cite the use of Epicentre’s Ribo-Zero™ Kit (Human/Mouse/Rat) to remove interfering ribosomal RNA from the experimental matrix, allowing closer study of the interactions between piRNAs. They also used the ScriptSeq™ mRNA-Seq Kit for an unusual application: sequencing small RNAs. Under normal circumstances, small RNAs <50-60 nucleotides are better suited for Epicentre's ScriptMiner™ Small RNA-Seq Kit, due to potential issues with the ScriptSeq method at the 5’ end of small RNAs. These results demonstrate that the ScriptSeq Kit has the ability to prepare RNA-Seq libraries from RNA types other than mRNA, including small RNAs.
Reuter, M. et al. (2011). Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing Nature, 480 (7376), 264-267 DOI: 10.1038/nature10672