Preparing high-quality DNA and RNA for Illumina sequencing

The MasterPure™ line of DNA and RNA purification products has become one of the tools of choice for researchers wishing to obtain pure, high-quality nucleic acids for deep sequencing and microarray-based analysis. The MasterPure procedure uses gentle detergent/salt and proteolysis-based extraction technology that does not require the use of toxic organics or spin-columns. The resulting high-quality nucleic acids--RNA (RIN > 9.5) and DNA (A_260/280 >1.8)--are of high molecular weight. Recent publications have highlighted the use of MasterPure kits to generate DNA for standard Illumina^® library construction and GAII sequencing for developing reference genomes and de novo deep sequencing of various genomes. Other successful applications using MasterPure-purified DNA and RNA include SNP detection, methylation analysis, and expression profiling.

Platform	MasterPure™ Kit	Sample Type	Reference
GAII	MasterPure Gram-Positive DNA Kit	Bacteria (Riemerella anatipestifer type)	Mavromatis, K et al. (2011) doi:10.4056/sigs.1553862
	MasterPure Gram-Positive DNA Kit	Bacteria (Bacteroides salanitronis)	Gronow S et al. (2011) doi:10.4056/sigs.1704212
	MasterPure Complete Kit	Bacteria (Geobacter sulfurreducens)	Nagarajan H et al. (2010) doi:10.1371/journal.pone.0010922
	MasterPure Gram-Positive Kit	Bacteria (Aminomonas paucivorans)	Pitluck S et al. (2010) doi:10.4056/sigs.1253298
	MasterPure Gram-Positive DNA Kit	Bacteria (Marivirga tractuosa)	Pagani, I et al. (2011) doi:10.4056/sigs.1623941
GoldenGate™	MasterPure DNA Purification Kit	Colorectal carcinoma lines	Irizarry, RA et al. (2008) doi: 10.1101/gr.7301508
BeadArray™	MasterPure DNA Purification Kit	Blood lymphocytes; cystic fibrosis patients	Darrah R et al. (2010) doi: 10.​1152/​physiolgenomics.​00185.​2009