The genomic sequencing libraries were created using the Nextera DNA Sample Prep Kit (Roche Titanium-compatible) to locate and characterize any viral entities that may be residing in the wild. The Nextera libraries did not require isolation of viroids from the individual and only a simple total genomic DNA preparation was needed to isolate sufficient DNA (50 ng) for library construction and sequencing. Analysis of the sequence data revealed that the two viral genotypes seen show greater evolutionary sequence divergence than has been observed in other coinfecting virions that infect individuals within a population. The authors state that the results of this investigation will increase the knowledge base of the history and biology of these pathogens, and demonstrate that wild primates are often reservoirs for novel pathogens.
Lauck, M. et al. (2011). Novel, Divergent Simian Hemorrhagic Fever Viruses in a Wild Ugandan Red Colobus Monkey Discovered Using Direct Pyrosequencing PLoS ONE, 6 (4) DOI: 10.1371/journal.pone.0019056