Thursday, August 4, 2011

Fosmid libraries aid in identifying a novel lipase

The CopyControl™ Fosmid Library Production Kit remains a key tool in the discovery and sourcing of novel, useful enzymes. In a recent publication, Glogauer et al. identified a novel lipase gene from a fosmid metagenomic library constructed with "prokaryotic-enriched" DNA from fat-contaminated soil collected from a wastewater treatment plant. The library, containing over 500,000 unique clones, revealed esterase activity in 32 of the library member clones after screening on agar plates containing 1% tricaprylin, or 1% triolein (denoting the presence of true lipases). One of these clones produced a novel lipase enzyme that the authors believe will be a useful tool in biocatalytic processes.

The researchers state that the novel lipase possesses “high specific activity against long-chain triacylglycerols, activity and stability over a wide range of pH values, good thermal stability, and stability in water-miscible organic solvents and at high salt concentrations.”

Thus, even with the growth of high-throughput sequencing technologies, CopyControl fosmid libraries continue to assist in the discovery of novel activities in metagenomic populations that may not be possible in high-copy-number cloning procedures.

ResearchBlogging.orgGlogauer, A. et al. (2011). Identification and characterization of a new true lipase isolated through metagenomic approach Microbial Cell Factories, 10 (1) DOI: 10.1186/1475-2859-10-54

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