Species compatibility for Ribo-Zero rRNA Removal Kits

Ribo-Zero™ rRNA Removal Kits are becoming the method of choice for RNA-Seq workflows, especially for degraded or compromised RNA samples. We often get questions from customers who want to know whether we have a Ribo-Zero kit for a particular organism. The information below offers some guidance on selecting the appropriate kit.

If you've used a Ribo-Zero kit for a species not listed here, e-mail your results to techhelp@epicentre.com, and we''ll send you a free Epicentre 2 GB flash drive!

Species	Ribo-Zero Kit (Human/Mouse/Rat) Compatibility
Human	>99% removal of 28S, 18S, 5.8S and 5S rRNAs
Mouse	>99% removal of 28S, 18S, 5.8S and 5S rRNAs
Rat	>99% removal of 28S, 18S, 5.8S and 5S rRNAs
Other mammalian (e.g., cow, pig)	Not tested but expected to perform well.
Drosophila	Will not remove 5S rRNA or 'right' fragment of 28S rRNA.
C. elegans	Use highest quality RNA possible.
Yeast	Removes 26S, 18S, 5.8S rRNAs from intact sample. Will not remove 5S rRNA. Use highest quality RNA possible.
Xenopus	Use highest quality RNA possible.
Mosquito	Not recommended
Zebra fish	Efficient removal of 28S, 18S, and 5S rRNAs. 5.8S rRNA removal has not been tested.
Lamprey	Use highest quality RNA possible.
Shrimp	Not recommended
Aspergillus niger	300 nt region of 18S rRNA will not be removed from a degraded sample. Use highest quality RNA possible.

Species	Ribo-Zero Kit (Gram-Negative Bacteria) Compatibility
E. coli	>99.9% removal of 23S, 16S and 5S rRNAs
Lactobacillus plantarum	>99% removal of 23S, 16S and 5S rRNAs
Salmonella typhimurium	Efficiently removes 16S and 5S rRNAs. Does not remove a 107 nt region of the fragmented 23S rRNA.
Campylobacter	Efficiently removes 23S and 16S rRNAs. Will not remove 5S rRNA.
Pseudomonas aeruginosa	Efficiently removes 23S, 16S and 5S rRNAs.
Porphyromonas gingivalis	Expected to efficiently remove 23S, 16S rRNAs. May not remove 5S rRNA.
Cyanobacteria	Efficiently removes 23S, 16S and 5S rRNAs.
Photorhabdus	Efficiently removes 23S, 16S and 5S rRNAs.
Francisella	Expected to efficiently remove 23S, 16S, 5S rRNAs.

Species	Ribo-Zero Kit (Gram-Positive Bacteria) Compatibility
Bacillus subtilis	Removes >99% of 23S, 16S and 5S rRNAs.
Mycobacterium tuberculosis	Efficiently removes 23S, 16S and 5S rRNAs.
Streptococcus	Expected to efficiently remove 23S, 16S, 5S rRNAs.
Clostridium difficile	Expected to efficiently remove 23S, 16S, 5S rRNAs.