Among 13 antibiotic-resistant clones, the authors isolated two genes that encode novel bifunctional proteins. One putative bifunctional protein confers resistance to ceftazidime and contains a natural fusion between a predicted transcriptional regulator and beta-lactamase. The ability to archive these activities in a genomic library enhances their future study under many different conditions.
Donato, J. et al. (2010). Metagenomic Analysis of Apple Orchard Soil Reveals Antibiotic Resistance Genes Encoding Predicted Bifunctional Proteins Applied and Environmental Microbiology, 76 (13), 4396-4401 DOI: 10.1128/AEM.01763-09