From this initial broad-host-range functional metagenomic study, Craig et al. demonstrate that some surrogate expression hosts will be better suited to using foreign genetic material than others. They conclude that continued exploration of phylogenetically diverse bacteria as hosts for functional metagenomic screening is likely to identify additional strains that will be suitable for eDNA functional metagenomics.
*Craig, J. et al. (2010). Expanding Small-Molecule Functional Metagenomics through Parallel Screening of Broad-Host-Range Cosmid Environmental DNA Libraries in Diverse Proteobacteria Applied and Environmental Microbiology, 76 (5), 1633-1641 DOI: 10.1128/AEM.02169-09