Thursday, September 9, 2010

Size distribution of Nextera Illumina-compatible libraries

Many of our Nextera™ customers have requested the ability to prepare libraries containing different sizes of DNA fragments for Illumina GAII sequencing. The Nextera™ DNA Sample Prep Kit (Illumina-compatible) now contains two buffers, Low-Molecular-Weight Buffer (LMW) and High-Molecular-Weight Buffer (HMW). The size distribution of the tagmented DNA can be controlled based on the buffer used in the reaction. Below are two Bioanalyzer traces from standard Nextera reactions using the indicated buffer.
LMW Buffer

HMW Buffer

We have also further optimized the reaction conditions to yield a narrow size distribution. For additional information on reaction conditions and on bias, library complexity, coverage, etc., please contact our technical support staff: techhelp@epibio.com

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