Tuesday, July 6, 2010

Gene expression analysis from Ribo-Zero™-treated RNA

The Ribo-Zero™ rRNA Removal Kit (Human/Mouse/Rat) was compared to a competitive kit's performance in removing rRNA from total RNA samples that were used for RNA-Seq. Both kits performed equivalently for intact RNA samples, while the Ribo-Zero Kit performed better than the competitive kit for partially degraded RNA samples.




Consistent gene expression data from Ribo-Zero™-treated RNA. Intact and partially degraded Universal Human Reference RNA (UHRR) was treated with either the Ribo-Zero Kit or a competitive kit. The rRNA-depleted RNAs were then used to prepare cDNA libraries that were sequenced on an Illumina® GAII sequencer.  A) Number of genes detected in libraries prepared from intact or partially degraded, rRNA-depleted UHRR. B) Correlation of expression levels for intact RNA. C) Correlation of expression levels for partially fragmented RNA. RPKM, reads per kilobase of exon model per million mapped reads.

(Click images to enlarge them.)

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