Tuesday, June 22, 2010

Superior ribosomal RNA (rRNA) removal for RNA-Seq

Epicentre recently launched the Ribo-Zero™ rRNA Removal Kit (Human/Mouse/Rat). The kit removes >99% of the 28S, 18S, and 5.8S and >95% of the 5S rRNA from both intact and partially degraded human, mouse, and rat total RNA preparations. The core procedure takes less than an hour; the rRNA-depleted samples can be purified either by ethanol precipitation or a column-based method. The kit provides an ideal solution for users who are preparing RNA-Seq libraries from human, mouse, or rat total RNA, especially from compromised samples such as formalin-fixed, paraffin-embedded (FFPE) tissue.

The Ribo-Zero™ rRNA Removal Kit (Human/Mouse/Rat) significantly improves RNA-Seq results. Intact and partially degraded Human Reference RNA was treated with either the Ribo-Zero Kit or a competitive rRNA removal kit. The rRNA-depleted RNA was then used to prepare RNA-Seq libraries that were sequenced on an Illumina® GAII sequencer.
Total RNA Sample rRNA Removal Kit % Ribosomal RNA Sequenced
Intact Human Reference RNA Ribo-Zero™ Kit 1.4%
Intact Human Reference RNA Competitive Kit 18.4%
Partially Degraded Human Reference RNA Ribo-Zero™ Kit 2.1%
Partially Degraded Human Reference RNA Competitive Kit 63.3%

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