Tuesday, May 18, 2010

Structural differences between Marburg and Ebola viruses

Ebola virus (EBOV) and Marburg virus (MARV) are related pathogens that cause hemorrhagic fevers. In many cases, viral infections are fatal. Both viruses are native to Africa where outbreaks have been occurring for decades. There is no effective therapy for the hemorrhagic fevers caused by these viruses. EBOV and MARV are in the same taxonomic family and are structurally identical; however, they elicit different antibodies. Enterlein et al.* used the AmpliScribe™ T7 High Yield Transcription Kit to investigate differences in RNA secondary structures between MARV and EBOV. They analyzed the structure of the MARV 3’-noncoding region and its influence on VP30. VP30 is an RNA binding protein which acts in trans with an RNA secondary structure upstream of the first transcriptional start site to modulate transcription.

The researchers choose the AmpliScribe system because it can efficiently transcribe RNA from limited amounts of DNA (as low as 1 ng). The AmpliScribe High Yield Transcription Kits can produce >20-fold more full-length RNA (both short and long transcripts) than conventional in vitro transcription reactions.

ResearchBlogging.orgEnterlein, S. et al. (2009). The Marburg Virus 3' Noncoding Region Structurally and Functionally Differs from That of Ebola Virus Journal of Virology, 83 (9), 4508-4519 DOI: 10.1128/JVI.02429-08

No comments: