Tuesday, April 20, 2010

Complete Genomics sequencing platform built on Epicentre’s enzymes

Last November, Complete Genomics published details of its third-generation sequencing platform that promises extremely high throughput at an estimated per-library cost of $4,400 for consumables.* The researchers sequenced three human genomes, generating an average of 45- to 87-fold coverage per genome and identifying 3.2 to 4.5 million sequence variants per genome.

The combinatorial probe-anchor ligation (cPAL) chemistry relies on the formation of DNA concatamers (termed “nanoballs”) that are clonally amplified from circular templates (see Complete Genomics workflow summary). The templates are prepared using Epicentre’s CircLigase ssDNA Ligase. In addition, the library preparation procedure used:
ResearchBlogging.org
*Drmanac, R. et al. (2009). Human Genome Sequencing Using Unchained Base Reads on Self-Assembling DNA Nanoarrays Science, 327 (5961), 78-81 DOI: 10.1126/science.1181498

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