Monday, February 22, 2010

Simplifying library preparation for next-generation sequencing

According to an industry research report on next-generation sequencing, published in August 2009:
  • The main bottleneck in sample preparation for next-gen sequencing reported was library construction.
  • The most used method for DNA fragmentation was nebulization for both short and medium/long fragments, and sonication for fragmentation for ChIP.
  • The aspect of sample prep for next-gen sequencing most in need of automation was DNA fragmentation.
Nextera™ technology was developed to address the concerns associated with these sample prep methods. DNA sample prep kits compatible with the Roche 454 and Illumina platforms were launched in early January. The kits use in vitro transposition technology to combine fragmentation and tagging of the DNA into a single-tube reaction. The technology will be showcased at the CHI XGen Congress, March 15-19, 2010 in San Diego, CA. A poster on applications of Nextera technology is also being presented this week at the 2010 AGBT meeting in Marco Island, FL.

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