The TotalScript Kit prepares high complexity RNA-Seq libraries from only 1-5 ng of intact total RNA, without the need for poly(A) enrichment or rRNA removal. Powered by Nextera™, TotalScript is index-capable, produces directional RNA-seq libraries, and is ideal for high quality RNA (RIN >7) from human, rodent, yeast, C. elegans and Drosophila. It is not recommended for prokaryotic samples.
The one-day procedure produces directional libraries, and is Index-capable (TotalScript™ Index kit sold separately). TotalScript can be adapted to desired rRNA content and transcript coverage based on chosen priming method.
Figure 1. Overview of the TotalScript™ RNA-Seq Kit process.